{"id":10,"date":"2023-08-09T13:31:21","date_gmt":"2023-08-09T17:31:21","guid":{"rendered":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/?page_id=10"},"modified":"2026-09-17T08:30:15","modified_gmt":"2026-09-17T12:30:15","slug":"methods","status":"publish","type":"page","link":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/methods\/","title":{"rendered":"Methods"},"content":{"rendered":"\n
\n\t\t\t\t\"how-heidi\"\n\t<\/figure>\n
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At the UNH Ecohydrology lab, we bring a wide variety of methodological approaches to bear on a variety of questions ranging from species-specific ecophysiology to ecosystem-scale evaluations of the impacts of forest management, climate variability, on water cycling and water stress.\u00a0 Our\u00a0signature methodologies\u00a0<\/em>which we have applied in a wide range of systems include:<\/p>\nPrecipitation manipulation experiments
\n<\/b>Experiments in forested ecosystems that allow the controlled and direct study of plant- and ecosystem-scale responses to water stress.\u00a0 Examples include the Northern Forest DroughtNet<\/a>\u00a0projects in New Hampshire and the\u00a0Wayqecha cloud forest drought experiment<\/a>\u00a0in Peru.\n

SELECTED PUBLICATIONS:<\/p>\n

Brum M<\/strong>, Deslauriers A, Vadeboncoeur MA, Frost TR, Moore DB,<\/strong> Rodrigues da Silva CB, Metcalfe DB, Asbjornsen H<\/strong>.\u00a0 Employing a hysteresis approach to analyze shifts in tree physiological thresholds in response to drought.\u00a0 Plant, Cell, & Environment<\/em>, 49: 4850-4871.\u00a0 https:\/\/doi.org\/10.1111\/pce.70498<\/a> \u00a0[open access]<\/p>\n

Anders E<\/strong>, Gotsch S, Vadeboncoeur M<\/strong>, Metcalfe D, Bartholomew D, Horwath A, Espinoza B, Galiano D, Asbjornsen H<\/strong>. 2025. Trait Plasticity and adaptive strategies of vascular epiphytes to a large-scale experimental reduction of fog immersion in a tropical montane cloud forest<\/u>. American Journal of Botany<\/em>, 112(5): e70042. https:\/\/doi.org\/10.1002\/ajb2.70042<\/a><\/p>\n

Brum M, Vadeboncoeur MA, Asbjornsen H<\/strong>, Puma Vilca BL, Galiano D, Horwath AB, Metcalfe DB.\u00a0 2023.\u00a0Ecophysiological controls on water use of tropical cloud forest trees in response to experimental drought<\/u>.\u00a0\u00a0Tree Physiology<\/i>, 43:1514-1532.\u00a0 doi: 10.1093\/treephys\/tpad070<\/a><\/p>\n

Asbjornsen H<\/strong>,\u00a0McIntire CD<\/strong>,\u00a0Vadeboncoeur MA<\/strong>,\u00a0Jennings KA<\/strong>,\u00a0Coble AP<\/strong>,\u00a0Berry ZC<\/strong>.\u00a0 2021.\u00a0\u00a0Sensitivity and threshold dynamics of\u00a0Pinus strobus\u00a0<\/em>and\u00a0Quercus\u00a0<\/em>spp. in response to experimental and naturally-occurring severe droughts<\/u>.\u00a0\u00a0Tree Physiology<\/em>, 41:1819-1835.\u00a0 doi: 10.1093\/treephys\/tpab056<\/a><\/p>\n

Asbjornsen H<\/strong>, Campbell JL,\u00a0Jennings KA<\/strong>,\u00a0McIntire CD<\/strong>,\u00a0Vadeboncoeur MA<\/strong>, Templer PH, Phillips R, Bauerle TL, Bowles F, Dietze M, Frey S, Groffman P, Guerrieri R, Hanson PJ, Kelsey E, Knapp AK, McDowell NG, Meir PW, Novick KA, Ollinger SV, Pockman W, Schaberg PG, Wullschleger SD, Smith MD, Rustad LE. 2018.\u00a0Guidelines and considerations for designing field experiments simulating precipitation extremes in forest ecosystems<\/u>.\u00a0Methods in Ecology and Evolution<\/i>, 9: 3210-2325. doi:10.1111\/2041-210X.13094<\/a>\u00a0\u00a0[PDF available from USFS<\/a>]<\/p>\n

\nSap flow sensors
\n<\/b>We employ both the heat-ratio and Granier approaches to measure vertical fluxes of water through trees.\u00a0\u00a0 We have deployed these types of sensors in a wide range of environments, and various species, sizes of trees.\nSELECTED PUBLICATIONS:
\nCoble AP<\/strong>, Contosta AR, Smith RG, Siegert NW,\u00a0Vadeboncoeur MA<\/strong>,\u00a0Jennings KA<\/strong>,\u00a0Stewart AJ<\/strong>,\u00a0Asbjornsen H<\/strong>. 2020.\u00a0Influence of forest-to-silvopasture conversion and drought on components of evapotranspiration<\/u>.\u00a0Agriculture, Ecosystems and Environment<\/i>, 295: 106916. doi:10.1016\/j.agee.2020.106916<\/a>\u00a0 [PDF available from USFS<\/a>]\n

McIntire CD<\/strong>, Huggett BA, Dunn E, Munck IA,\u00a0Vadeboncoeur MA<\/strong>,\u00a0Asbjornsen H<\/strong>.\u00a0\u00a0Pathogen-induced defoliation impacts on transpiration, leaf gas exchange, and non-structural carbohydrate allocation in eastern white pine (Pinus strobus<\/em>)<\/u>.\u00a0\u00a0Trees – Structure and Function<\/em>, in press.\u00a0 doi:\u00a010.1007\/s00468-020-02037-z<\/a><\/p>\n

Guti\u00e9rrez L\u00f3pez JA<\/strong>, Licata J, Pypker T,\u00a0Asbjornsen H<\/strong>. 2019.\u00a0Effects of heater wattage on sap flux density estimates using an improved tree-cut experiment<\/u>.\u00a0Tree Physiology<\/i>. 39: 679-693. doi:10.1093\/treephys\/tpy137<\/a><\/p>\n

Sinacore\u00a0<\/strong>K,\u00a0Asbjornsen H<\/strong>,\u00a0Hernandez Santana V<\/strong>, Hall JS. 2019.\u00a0Drought differentially affects growth, transpiration, and water use efficiency of mixed and monospecific planted forests<\/u>.\u00a0Forests<\/i>\u00a010: 153. doi:10.3390\/f10020153v (open access)<\/a><\/p>\n

Brum M,\u00a0Guti\u00e9rrez L\u00f3pez JA<\/strong>,\u00a0Asbjornsen H<\/strong>, Licata J, Pypker T, Sanchez G, Oiveira RS. 2018.\u00a0ENSO effects on the transpiration of eastern Amazon trees<\/u>.\u00a0Philosophical Transactions of the Royal Society B – Biological Sciences\u00a0<\/i>373: 20180085. doi:10.1098\/rstb.2018.0085<\/a><\/p>\n

\n\u00a0Isotopic dendrochronology
\n<\/strong>This approach enables analyses of interannual variability and long-term trends in the carbon and water relations of trees based on the width and stable isotope ratios of tree rings.\nSELECTED PUBLICATIONS:
\n\n

Malcomb, JD, Scanlon TM, Epstein HE, Vadeboncoeur MA<\/strong>, Druckenbrod DL, Lanning M, Wang L, Asbjornsen H<\/strong>. 2025. Divergent water use efficiency trends among eastern North American temperate tree species<\/u>. Oecologia<\/em>, 207:137. https:\/\/doi.org\/10.1007\/s00442-025-05753-w<\/a><\/p>\n

Vadeboncoeur MA<\/strong>,\u00a0Jennings KA<\/strong>, Ouimette AP,\u00a0Asbjornsen H<\/strong>. 2020.\u00a0Correcting tree-ring \u1e9f13<\/sup>C time series for tree-size effects in eight temperate tree species<\/u>.\u00a0Tree Physiology<\/i>, 40: 333- 349. doi:\u00a010.1093\/treephys\/tpz138<\/a><\/p>\n

Guerrieri R<\/strong>,\u00a0Jennings K<\/strong>, Belmecheri S,\u00a0Asbjornsen H<\/strong>, Ollinger S. 2017.\u00a0Evaluating climate signal recorded in tree-ring \u03b413<\/sup>C and \u03b418<\/sup>O values from bulk wood and \u03b1-cellulose for six species across four sites in the northeastern US<\/u>.\u00a0Rapid Commununications in Mass Spectrometry<\/i>. 31: 2081-2091. doi:10.1002\/rcm.7995<\/a><\/p>\n

Jennings KA<\/strong>,\u00a0Guerrieri R<\/strong>,\u00a0Vadeboncoeur MA<\/strong>,\u00a0Asbjornsen H<\/strong>. 2016.\u00a0Response of\u00a0Quercus velutina\u00a0<\/i>growth and water use efficiency to climate variability and nitrogen fertilization in a temperate deciduous forest in the northeastern U.S<\/u>.\u00a0Tree Physiology<\/i>, 36:428-443. doi:10.1093\/treephys\/tpw003<\/a><\/p>\n

We are always interested in collaborative opportunities to apply these approaches to interesting ecological, physiological, and global-change questions in a variety of ecosystem types.<\/p>\n\n","protected":false},"excerpt":{"rendered":"

At the UNH Ecohydrology lab, we bring a wide variety of methodological approaches to bear on a variety of questions ranging from species-specific ecophysiology to ecosystem-scale evaluations of the impacts of forest management, climate variability, on water cycling and water stress.\u00a0 Our\u00a0signature methodologies\u00a0which we have applied in a wide range of systems include: Precipitation manipulation […]<\/p>\n","protected":false},"author":171,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-10","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/pages\/10","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/users\/171"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/comments?post=10"}],"version-history":[{"count":7,"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/pages\/10\/revisions"}],"predecessor-version":[{"id":277,"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/pages\/10\/revisions\/277"}],"wp:attachment":[{"href":"https:\/\/sites.usnh.edu\/ecohydrology-lab\/wp-json\/wp\/v2\/media?parent=10"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}